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Günter, Elena A Shkryl, Yury N Popeyko, Oxana V Veremeichik, Galina N Bulgakov, Victor P Although the biochemical functions of rol genes remain poorly understood, they are useful tools for improving ornamental flowers, as their expression in transgenic plants yields many beneficial traits.Ĭell-wall polysaccharide composition and glycanase activity of Silene vulgaris callus transformed with rolB and rolC genes. rolD, the least studied among the rol genes, offers promising applications due to its promotion of flowering. In addition to the dwarfness and the increase in lateral shoots that lead to a bushy phenotype, rolC-plants display more, smaller flowers, and advanced flowering surprisingly, these plants may have better rooting capacity and they show almost no undesirable traits. Among the rol genes, termed rolA, B, C and D, rolC has been the most widely studied because its effects are the most advantageous in terms of improving ornamental and horticultural traits. In general, these plants show a dwarfed phenotype, reduced apical dominance, smaller, wrinkled leaves, increased rooting, altered flowering and reduced fertility. rhizogenes- and rol-transgenic plants display the "hairy-root phenotype", although specific differences are found between species and between transgenic lines. Overexpression of the rol genes of the Ri plasmid of Agrobacterium rhizogenes in plants alters several of the plant's developmental processes and affects their architecture. New or improved varieties of floricultural crops can be obtained by acting on floral traits, such as color, shape or fragrance, on vase life in cut-flower species, and on rooting potential or overall plant morphology. However, genetic engineering now enables specific alterations of single traits in already successful varieties. Traditionally, new traits have been introduced into ornamental plants through classical breeding. Casanova, Eva Trillas, Maria Isabel Moysset, Lluïsa Vainstein, Alexander